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Cell line:K-562
DSMZ no.:ACC 10
Species:human (Homo sapiens)
Cell type:chronic myeloid leukemia in blast crisis
Origin:
established from the pleural effusion of a 53-year-old woman with chronic myeloid leukemia (CML) in blast crisis in 1970; cells carry the BCR-ABL1 e14-a2 (b3-a2) fusion gene; this subclone (ACC 10) weakly expresses MHC class I (image); other subclones (ACC 86 and ACC 92) are MHC class I negative (image) and might be used in in-vitro natural killer assays; cells produce hemoglobin
Biosafety level:1
Permissions and restrictions:A
DSMZ Cell Culture Data:
Morphology:round large, single cells in suspension;
Medium:90% RPMI 1640 + 10%FBS
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Subculture:
maintain at 0.1-0.5 x 106 cells/ml; split 1:3 to 1:5 every 3 days; seed out at ca. 0.3-0.5 x 106 cells/ml; viability may be low after thawing, but cells recover during the following 2-3 days, culture should be started with 20% FBS in 24-well-plates.
Incubation:at 37 °C with 5% CO2
Doubling time:ca. 30-40 hours
Harvest:maximal density at 1.0-1.5 x 106 cells/ml
Storage:frozen with 70% medium, 20% FBS, 10% DMSO
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