OptiPrep™ is a sterile and endotoxin tested solu- tion of 60% iodixanol in water with a density of 1.32 g/ml.
Iodixanol was developed as an X-ray contrast me- dium an has therefore been subjected to rigorous clinical testing.
Iodixanol is non-ionic, non-toxic to cells and met- abolically inert.
Iodixanol solutions can be made isoosmotic at all useful densities.
Iodixanol solutions have low viscosity and osmo- lality.
The high density of OptiPrep™ facilitates the fractionation of cells by flotation from a dense load zone through either a continuous or discontinuous gradient or through a simple density barrier.
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51 protocols available for cell isolation.
Improved resolution of cell organelles.
Low viscosity, isoosmotic gradients provide rapid and efficient separation of the major organelles in preformed gradients.
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62 protocols available for isolation of subcellu- lar organelles and membranes.
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OptiPrep™ is the ideal solution for virus purification
Virus purified using OptiPrep™ gradients shows infectivity: particle number ratios at least 100x
those from CsCl gradients.
38 protocols available for virus purificatio
13 protocols available for macromolecules and lipoproteins purification.
OptiPrep™ is a ready-made, sterile and endotoxin tested solution with the following specifications:
Iodixanol: 60% (w/v)
Density: 1.320 ± 0.001 g/ml Endotoxins: < 1.0 EU/ml
Each batch of OptiPrep™ is checked on the level of endotoxins using a specific LAL test. Our goal
is to produce batches with an endotoxin level lower or equal to 0.13 EU/ml.
For every batch produced a Certificate of Analysis showing the actual values of density and endotoxin levels is made available at www.axis-shield- density-gradient-media.com. We also claim sterili- ty according to Ph.Eur.
OptiPrep™ is manufactured, packed and released in compliance with ISO 13485
OptiPrep™ is supplied as a sterile solution in the following package size:
Prod. no. 1114542 1x250ml
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Mammalian and non-mammalian cells
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Subcellular organelles
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Analysis of membrane trafficking and cell signal- ling
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Analysis of endocytosis and exocytosis
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Plasma membrane and domains
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Membrane vesicles and cytosol
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Organelles from non-mammalian sources
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Viruses
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Plasma lipoproteins
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Proteins and protein complexes
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Plasmid DNA
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Ribonucleoproteins
